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New mobile tool analyzes microbes in watery environs
by Brooks Hays
Washington DC (UPI) Jan 27, 2020

Glass frogs reappear in Bolivia after 18 years
La Paz (AFP) Jan 27, 2020 - A rare species of frog native to the eastern slopes of the Bolivian Andes has been spotted in the South American country for the first time in 18 years, the investigation team that made the discovery told AFP.

The Bolivian Cochran frog is notable for its transparent belly, leading to its nickname, the "glass frog".

"The rediscovery of this species fills us with a ray of hope for the future of the glass frogs -- one of the most charismatic amphibians in the world -- but also for other species," said investigation team members Rodrigo Aguayo and Oliver Quinteros, from the Natural History Museum "Alcide d'Orbigny", and Rene Carpio of the San Simon University in Cochabamba.

The team came across the frogs on January 8 during a mission to rescue reptiles and amphibians threatened by a hydroelectric project in the Carrasco National Park to the east of Cochabamba, the fourth largest city in Bolivia.

Glass frogs are tiny, measuring only 0.7-0.9 inches (19-24 millimeters) and weighing just 2.5-2.8 ounces (70-80 grams). They can be found in the departments of La Paz (west), Cochabamba, Santa Cruz (east) and Chuquisaca (southeast).

Some frogs' hearts and digestive tracts can be seen through their transparent bellies.

Those found in the Carrasco National Park had a transparent belly with a "white chest. The bones and vocal sac of the males are dark green," the team said.

The three frogs found were taken to the K'ayra amphibian conservation center at the Alcide d'Orbigny museum.

Experts will try to encourage the frogs to breed as part of a conservation strategy.

The K'ayra Center is also home to a pair of Sehuenca water frogs, known as Romeo and Juliet, that scientists have been trying to convince to mate to help preserve their critically endangered species.

Their attempts have so far been in vain.

Scientists at Rutgers University have developed a small, mobile device capable of quickly analyzing microbes present in a variety of watery environments.

The technology was originally designed to study algae and its relationship with coral. The device, which can be used in the lab or in the field, can measure the effects of environmental stressors, like pollution or increases in water temperature, on microbes and cells.

Scientists described the new device in a paper published Monday in the journal Scientific Reports.

"This is very important for environmental biology, given the effects of climate change and other stressors on the health of microorganisms, such as algae that form harmful blooms, in the ecosystem," study author Mehdi Javanmard, an expert in nanotechnology and an associate professor of electrical and computer engineering at Rutgers University-New Brunswick, said in a news release.

Researchers wanted a way to quickly observe the health of cells in ocean samples without deploying big, expensive technologies like genomic sequencing tools.

"Being able to assess and understand the status of cells, without having to send samples back to the lab, can allow the identification of threatened ecosystems based on a 'stress index' for their inhabitants," said co-author Debashish Bhattacharya, a distinguished professor in the Department of biochemistry and microbiology at Rutgers.

To test the new device, scientists used Picochlorum, a common green microalga, as the subject. Scientists deployed the technology while subjecting microalga cells to a variety of environmental conditions. The tool successfully determined whether the cells were stressed, energized or unaffected.

The tool features tiny channels, through which microbes pass one-by-one. As they pass through the micro-channel, a sensor measures the disruption of the device's electrical field, or impedance, which offers clues to the cell's size and physiological state.

"Our results demonstrate the utility of electrical impedance as an indicator of cell phenotype by providing results that are consistent with known changes in cell size and physiology," researchers wrote in their paper.

In addition to studying algae, the technology could also be used to screen for food-borne pathogenic bacteria, antibiotic resistant bacteria and other harmful microbes.


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FLORA AND FAUNA
Scientists to search for relatives of extinct Galapagos tortoises
Quito (AFP) Jan 25, 2020
A scientific expedition to the Galapagos Islands will spend ten days searching for relatives of two tortoise species believed to be extinct, including those of the archipelago's Lonesome George, park officials said Friday. George, the last known member of the Pinta tortoise species, died in 2012 at over 100 years of age after refusing to mate in captivity with females from related subspecies. Park rangers and scientists from the Galapagos National Parks (PNG) and Galapagos Conservancy will tour ... read more

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