Transporting methane from gas wellheads to market provides multiple opportunities for this greenhouse gas to leak into the atmosphere. Now, an international team of researchers has taken the first step in converting methane directly to electricity using bacteria, in a way that could be done near the drilling sites.

"Currently, we have to ship methane via pipelines," said Thomas K. Wood, holder of the biotechnology endowed chair and professor of chemical engineering, Penn State. "When you ship methane, you release a greenhouse gas. We can't eliminate all the leakage, but we could cut it in half if we didn't ship it via pipe long distances."

The researchers' goal is to use microbial fuel cells to convert methane into electricity near the wellheads, eliminating long-distance transport. That goal is still far in the future, but they now have created a bacteria-powered fuel cell that can convert the methane into small amounts of electricity.

"People have tried for decades to directly convert methane," said Wood. "But they haven't been able to do it with microbial fuel cells. We've engineered a strain of bacteria that can."

Microbial fuel cells convert chemical energy to electrical energy using microorganisms. They can run on most organic material, including wastewater, acetate and brewing waste. Methane, however, causes some problems for microbial fuel cells because, while there are bacteria that consume methane, they live in the depths of the ocean and are not currently culturable in the laboratory.

"We know of a bacterium that can produce an energy enzyme that grabs methane," said Wood. "We can't grow them in captivity, but we looked at the DNA and found something from the bottom of the Black Sea and synthesized it."

The researchers actually created a consortium of bacteria that produces electricity because each bacterium does its portion of the job. Using synthetic biological approaches, including DNA cloning, the researchers created a bacterium like those in the depths of the Black Sea, but one they can grow in the laboratory. This bacterium uses methane and produces acetate, electrons and the energy enzyme that grabs electrons.

The researchers also added a mixture of bacteria found in sludge from an anaerobic digester - the last step in waste treatment. This sludge contains bacteria that produce compounds that can transport electrons to an electrode, but these bacteria needed to be acclimated to methane to survive in the fuel cell. They report the results of their work today (May 17) in Nature Communications.

"We need electron shuttles in this process," said Wood. "Bacteria in sludge act as those shuttles."

Once electrons reach an electrode, the flow of electrons produces electricity. To increase the amount of electricity produced, the researchers used a naturally occurring bacterial genus - Geobacter, which consumes the acetate created by the synthetic bacteria that captures methane to produce electrons.

To show that an electron shuttle was necessary, the researchers ran the fuel cell with only the synthetic bacteria and Geobacter. The fuel cell produced no electricity. They added humic acids - a non-living electron shuttle - and the fuel cells worked. Bacteria from the sludge are better shuttles than humic acids because they are self-sustaining. The researchers have filed provisional patents on this process.

"This process makes a lot of electricity for a microbial fuel cell," said Wood. "However, at this point that amount is 1,000 times less than the electricity produced by a methanol fuel cell."

Tweet

Genome sequence of fuel-producing alga announced
College Station TX (SPX) May 15, 2017
The genome of the fuel-producing green microalga Botryococcus braunii has been sequenced by a team of researchers led by a group at Texas A and M AgriLife Research. The report, in Genome Announcements, comes after almost seven years of research, according to Dr. Tim Devarenne, AgriLife Research biochemist and principal investigator in College Station. In addition to sequencing the genome, ... read more

Related Links
Penn State
Bio Fuel Technology and Application News

Thanks for being here; We need your help. The SpaceDaily news network continues to grow but revenues have never been harder to maintain. With the rise of Ad Blockers, and Facebook - our traditional revenue sources via quality network advertising continues to decline. And unlike so many other news sites, we don't have a paywall - with those annoying usernames and passwords. Our news coverage takes time and effort to publish 365 days a year. If you find our news sites informative and useful then please consider becoming a regular supporter or for now make a one off contribution.
SpaceDaily Contributor $5 Billed Once credit card or paypal		SpaceDaily Monthly Supporter $5 Billed Monthly paypal only